Burhana Mawarasti*, Herry Purbayu**, Lindawati Alimsardjono***

*Faculty of Medicine, Universitas Airlangga, Surabaya

**Division of Gastroentero-hepatology, Department of Internal Medicine Universitas Airlangga/Dr. Soetomo General Hospital, Surabaya

***Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya

Corresponding author: Herry Purbayu. Division of Gastroentero-hepatology, Department of Internal Medicine, Dr. Soetomo General Hospital. Jl. Mayjend Prof Dr Moestopo No.6-8 Surabaya Indonesia. Phone/facsimile: +62-31-5501614. Email: h_purbayu@yahoo.com

ABSTRACT

Background: Helicobacter pylori (H. pylori) is known as the main pathogen which causes infection in human’s stomach. There are three strains of H. pylori, which are type I, intermediate, and type II strains of H. pylori. Strain type I has cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene A (vacA) while strain type II has only vacuolating cytotoxin gene A (vacA). The aim of this study is to determine whether the stool sample shows cagA gene and or vacA gene expression. The benefit of this study is to understand the strains of H. pylori in order to prevent misdiagnosis and recurrent infection.

Method: This study was conducted by using descriptive purposeful sampling method upon patients in Endoscopy Department of Internal Medicine in the Division of Hepatology Gastroentero Dr. Soetomo starting from 20 October to 25 November 2015. Total patients in this study were ten patients each was selected by random sampling. The aim of this study is to determine whether the stool sample shows cagA gene and or vacA gene expression. The data was processed by observing results of polymerase chain reaction (PCR) assays to look at the genes that were expressed by H. pylori. DNA was extracted from stool by using QIAamp (Qiagen) stool kit.

Results:Results of the study showed only one patient was positive for vacA gene while cagA gene was found in none of those ten patients. DNA examinations with different concentrations and temperatures also showed similar results. One sample from the stool specimens showed positive for type II strain, indicating that it only had vacA gene. PCR examination through gastric biopsy has been known for its high specificity.

Conclusion: In polymerase chain reaction (PCR) examination of extracted gene from patients’ faecal specimen, cagA gene expression which was type I strain of H. pylori was not found. Meanwhile, vacA gene expression was found to be positive in one patient which indicated type II strain of H. pylori.

Keywords: Helicobacter pylori, cagA, vacA, polymerase chain reaction (PCR), stool specimen

Indones J Gastroenterol Hepatol Dig Endosc Vol.17, No.3, December 2016 (FULL TEXT)